FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181460
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT2770.1526507219221867No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA201.0459632E-466.160382
TACATAC150.002778808166.160372
GACTCGT200.0066044553.16563443
CTCCGTT400.006662719453.0247470
CTCTTAT253.1521925E-452.92831
CTTATAC1200.052.376961
TACCCTA200.00867714949.620286
CTACATA200.00867714949.620281
AGGGGGG1153.0468073E-949.1823670
TATACAC1300.048.3479653
AGGAGTA307.745813E-444.1069152
ATACACA1500.041.901574
TACACAT1600.041.3502355
TCTGCTT653.607256E-739.12907453
AGACGTA1550.038.5431525
CTCGTAT556.9383186E-637.95984339
TTTAATT350.001642131837.8580922
AACAAAA501.18383934E-437.23573362
ACACATC1700.036.9719736
TTATACA1700.036.9719732
Produced by FastQC (version 0.11.5)