FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041294
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTCG306.5306196E-445.6820265
GTATCAA29350.040.380371
GGTATCA12950.037.2950861
ATCAACG35350.033.8188743
TCAACGC35850.033.3472064
CAACGCA36350.032.8948255
TATCAAC36650.032.711192
AACGCAG37150.032.2786836
ACGCAGA40250.029.703227
AGAGTAC41000.028.5736311
CAGAGTA41350.028.41461210
CGCAGAG43200.027.7541738
TTCGTTA500.0080158627.39868414
GCAGAGT44200.026.9712439
GTGGTAT5050.025.0964681
GAGTACG33600.024.768912
AGTACGG33800.024.72366513
CGAATTA1251.3570389E-724.65881515
TAGAGCG851.5127122E-424.18465
TGGTATC5550.024.0698782
Produced by FastQC (version 0.11.5)