FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160926
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.11654489605711303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66150.039.905651
GTGGTAT9750.039.1730161
CTTATAC3950.035.938951
TATCAAC75350.035.257582
TATACAC4650.034.1631663
ATCAACG80350.033.0215153
TCGTATG2000.032.89034740
GGTATCA29450.032.4798741
TCAACGC82050.032.4197274
CAACGCA81950.032.37685
AACGCAG83350.031.7532426
TTATACA4950.031.409852
CGAGACA4550.030.47002223
ACGAGAC4650.029.81089822
ACGCAGA91200.029.0546557
TGGTATC13550.028.9355582
CGTATGC2400.028.9175341
CGCAGAG92300.028.6717768
AGAGTAC93100.028.02606611
CAGAGTA93950.027.88042810
Produced by FastQC (version 0.11.5)