FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063079
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19220.18079559468299156No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12420.11683045192314025No Hit
GATCTATGCTGCATCCACTTTACAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGCACAGATTTCA11390.10714161412275099No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11330.10657721580428171No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10930.10281456034781987No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10760.1012154317788236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25450.041.709491
TATCAAC29000.036.6036762
ACGTTAG901.4699476E-435.90996670
TATACCG601.3818513E-534.354586
TCAACGC30750.034.2971044
ATCAACG31150.033.9669763
CAACGCA32050.032.9075055
AACGCAG33750.031.2499456
ACGCAGA36000.029.5817187
ATACCGC703.9646053E-529.4453987
AGAGTAC37050.028.55792811
CGCAGAG37200.028.4427748
CAGAGTA37300.028.36652410
GCAGAGT39600.026.8057219
AACCCGA2008.861007E-725.85517370
CTAAGGT951.0860564E-525.3127124
GAGTACG27500.024.48314312
AGTACGG27500.024.48314313
GGTATCA17500.024.3415281
TATATAC1852.0008883E-1124.139924
Produced by FastQC (version 0.11.5)