FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1229492
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG750.055.4152540
CTCGTAT1050.039.5352439
GTATCAA37300.037.1529351
CGTATGC1151.8189894E-1236.1700941
GGTATCA17850.035.5511861
TCAACGC43700.031.7928544
ATCAACG43750.031.6755313
TATCAAC44050.031.4598052
CAACGCA44300.031.2848135
ACGTAGA2600.030.38988927
AGACGTA2600.030.3750425
AACGCAG45550.030.2768886
ACGCAGA49100.028.085547
CGTAGAG2850.027.73427628
CGAGACG3000.027.45618823
CGCAGAG50850.027.051528
AGAGTAC50200.026.98959211
GTGGTAT6900.026.8451141
TCTCGTA1555.0931703E-1126.75998338
CAGAGTA51000.026.7029110
Produced by FastQC (version 0.11.5)