FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263978
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTAATGGTCAGGGAGGCTGTAATCCCTGACCTGGATGCAGAGAAT51530.4076811463490662No Hit
TTACTGGACTGCAGCCTGAGGACGAGGCTGACTATTACTGCTCAACATGG43330.342806599481953No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCAGTCCAGTAATGGTCAGGGAGG42470.3360026835910119No Hit
GTCCCATGTTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCAGTCCAGTAATGGTCAGGGAGGCTGTAATCC41890.3314139961296795No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39530.31274278508012004No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39450.3121098626716604No Hit
ACATGGGACAGCAGCCTCAGTGTTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG38560.3050686008775469No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31790.2515075420616498No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31340.24794735351406436No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31240.2471562005034898No Hit
GACTATTACTGCTCAACATGGGACAGCAGCCTCAGTGTTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTA31140.24636504749291524No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30110.23821617148399735No Hit
CACCTGCACTGGAAACAGCAGCAATGTTGGCGACCGAGGAGCAGTTTGGCTGCAGCAGCACCAGGGCCACCCTCC29690.23489332883958425No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26450.20925997129696877No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC26160.20696562756630257No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26090.20641182045890039No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT25880.20475039913669382No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGC25880.20475039913669382No Hit
GTCCAAGGGCTTGAGGCAGACCGCCACACTCACCTGCACTGGAAACAGCA25130.1988167515573847No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24440.1933577957844203No Hit
CCACAACACTGAGGCTGCTGTCCCATGTTGAGCAGTAATAGTCAGCCTCG24300.19225018156961593No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23670.18726591760299627No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23310.18441776676492788No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23040.18228165363637658No Hit
GTGTTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTACGTCAGCCC22690.17951261809936564No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22350.17682269786341218No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22300.1764271213581249No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA22230.17587331425072272No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22200.17563596834755035No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22030.1742910082295736No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21870.17302516341265434No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21720.17183843389679251No Hit
GACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACAACACTGAGG21460.16978143606929869No Hit
CCGCCACACTCACCTGCACTGGAAACAGCAGCAATGTTGGCGACCGAGGA21340.1688320524566092No Hit
GTCCAGGCAGGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGGCA20960.1658256710164259No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20180.1596546775339444No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGGCAGACCGCCAC20170.15957556223288696No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19800.15664829609376113No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19730.15609448898635894No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19380.153325453449348No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19340.15300899224511819No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC18880.14936968839647527No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA18640.14747092117109634No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC18450.1459677304510047No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18440.14588861514994722No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18330.14501834683831522No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18140.14351515611822357No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18040.14272400310764904No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCAC17890.1415372735917872No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17560.13892646865689118No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG17530.1386891227537188No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17520.13861000745266136No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17470.13821443094737407No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG17110.1353662801093057No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16800.1329137057765246No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16270.12872059482047946No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG16120.12753386530461763No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15880.12563509807923873No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC15880.12563509807923873No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15810.12508129097183654No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15710.12429013796126198No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15550.12302429314434271No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15470.12239137073588306No Hit
GGCTTGAGGCAGACCGCCACACTCACCTGCACTGGAAACAGCAGCAATGTTGGCGACCGAGGAGCAGTTTGGCTG15460.1223122554348256No Hit
CTCCTGTACAACTTCTGGATTCACGTTTGGCGACTATGTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG15220.12041348820944668No Hit
AGGCAGGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGGCAGACC14690.11622037725340156No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14690.11622037725340156No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14660.1159830313502292No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG14580.11535010894176956No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14280.1129766499100459No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14270.11289753460898845No Hit
CCCTGAGACTCTCCTGTACAACTTCTGGATTCACGTTTGGCGACTATGTT14220.11250195810370119No Hit
TCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGGCAGGGCTG14070.11131522858783935No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13960.11044496027620733No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13870.10973292256669025No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13870.10973292256669025No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG13830.10941646136246042No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13810.10925823076034552No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13790.10910000015823061No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC13740.10870442365294333No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13730.10862530835188586No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13660.1080715012444837No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13500.1068056564275644No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA13430.10625184932016223No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13370.10577715751381749No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAT13350.10561892691170256No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13170.10419485149266838No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13080.10348281378315129No Hit
GTAATGGTCAGGGAGGCTGTAATCCCTGACCTGGATGCAGAGAATCTCTC13050.10324546787997893No Hit
GTTTTCAGGCTGTCCATTTGCAGATAGGCGACACTTTTGGAATCATCTCT12990.10277077607363419No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12980.10269166077257674No Hit
CAGCAGCACCAGGGCCACCCTCCCAAACTCCTGTCCTACAGGAATAACAA12750.10087200884825527No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12740.10079289354719782No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12730.10071377824614036No Hit
GTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTGG12700.10047643234296799No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12690.10039731704191054No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC12640.10000174053662328No Hit
ACAGAATACGCCGCGTCCGTGAAAGGCAGATTCACCATTTCAAGAGATGA12640.10000174053662328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22100.041.4952621
TGGTATC2850.040.9744952
GGTATCA8600.040.336891
CTTATAC1400.039.2528721
TACACAT1550.037.674575
TTATACA1550.037.6700972
GGCGGTT22250.036.0112970
TATCAAC26100.035.6622122
CGCTAAT1500.034.4430232
TCGCTAA1600.034.42936331
CGTATGC1450.033.4437641
TCGTATG1350.033.33400340
CTCGTAT1154.5474735E-1133.0833339
AACGCAG28650.032.4919476
TATACAC1800.032.439423
ATCAACG28600.032.305983
CAACGCA29000.032.0998045
TCAACGC28800.032.0816354
TTCGGGG10750.031.58262870
GGCGTAT7100.030.60401370
Produced by FastQC (version 0.11.5)