FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263978
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGTAATGGTCAGGGAGGCTGTAATCCCTGACCTGGATGCAGAGAAT48370.38268071121491043No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46570.36843995702456844No Hit
GTCCCATGTTGAGCAGTAATAGTCAGCCTCGTCCTCAGGCTGCAGTCCAGTAATGGTCAGGGAGGCTGTAATCC42040.33260072564554133No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41740.3302272666138176No Hit
TTACTGGACTGCAGCCTGAGGACGAGGCTGACTATTACTGCTCAACATGG39040.30886613532830476No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGCAGTCCAGTAATGGTCAGGGAGG38110.30150841232996145No Hit
ACATGGGACAGCAGCCTCAGTGTTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGG36400.28797969584913663No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG32920.26044757108114225No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31790.2515075420616498No Hit
GACTATTACTGCTCAACATGGGACAGCAGCCTCAGTGTTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTA29680.23481421353852677No Hit
GACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACAACACTGAGG28790.22777295174441328No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28330.22413364789577034No Hit
CACCTGCACTGGAAACAGCAGCAATGTTGGCGACCGAGGAGCAGTTTGGCTGCAGCAGCACCAGGGCCACCCTCC27730.21938672983232302No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26030.20593712865255567No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25700.20332632371765966No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGC25660.2030098625134298No Hit
GTGTTGTGGTATTCGGCGGCGGGACCAAGCTGACCGTCCTACGTCAGCCC24830.19644329252566103No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24290.19217106626855845No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24070.19043052964529447No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23940.18940203073154754No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23870.18884822362414536No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC23600.18671211049559405No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCAC23470.18568361158184715No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG23420.1852880350765599No Hit
GTCCAAGGGCTTGAGGCAGACCGCCACACTCACCTGCACTGGAAACAGCA23400.18512980447444496No Hit
CCACAACACTGAGGCTGCTGTCCCATGTTGAGCAGTAATAGTCAGCCTCG22930.18141138532474457No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22740.17990819460465293No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22560.17848411918561874No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22410.1772973896697569No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21980.17389543172428634No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21430.16954409016612632No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21300.1685155912523794No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21060.16661682402700045No Hit
GGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGGCAGACCGCCAC21050.166537708725943No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20930.16558832511325355No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20920.1655092098121961No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20310.16068317644769134No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG20160.15949644693182952No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT19860.15712298790010584No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT19570.15482864416943967No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA19380.153325453449348No Hit
GTCCAGGCAGGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGGCA19320.1528507616430033No Hit
CCGCCACACTCACCTGCACTGGAAACAGCAGCAATGTTGGCGACCGAGGA19120.15126845562185418No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18740.14826207418167087No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT18730.14818295888061342No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18460.14604684575206214No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT18290.14470188563408543No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17440.13797708504420172No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA17360.1373441626357421No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG17350.13726504733468464No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17290.13679035552833987No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA17240.1363947790230526No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16940.13402131999132896No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16930.13394220469027152No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC16900.13370485878709915No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16530.13077759264797328No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16300.12895794072365183No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16010.12666359699298563No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15750.12460659916549181No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC15670.12397367675703218No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC15520.12278694724117034No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA15360.12152110242425104No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15340.12136287182213615No Hit
GGCTTGAGGCAGACCGCCACACTCACCTGCACTGGAAACAGCAGCAATGTTGGCGACCGAGGAGCAGTTTGGCTG15100.11946410459675721No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14960.11835649038195285No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG14890.11780268327455067No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14650.11590391604917175No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14640.11582480074811428No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14630.11574568544705684No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG14500.11471718653330992No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14190.1122646122005288No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14050.11115699798572444No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13890.10989115316880516No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13790.10910000015823061No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG13700.1083879624487135No Hit
GGGCTGGAGTGGGTTGGTTTGATTAGAAGCAAAACTTATGGTGGGACAAC13540.10712211763179423No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA13500.1068056564275644No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13460.10648919522333458No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13310.10530246570747276No Hit
AGGCAGGGCTGACTCAGCCACCCTCGGTGTCCAAGGGCTTGAGGCAGACC13230.10466954329901312No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTCTCTTGTCC13220.10459042799795565No Hit
CTCCTGTACAACTTCTGGATTCACGTTTGGCGACTATGTTATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13190.10435308209478329No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC13120.1037992749873811No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12920.10221696896623202No Hit
ACGTAGGACGGTCAGCTTGGTCCCGCCGCCGAATACCACAACACTGAGGC12920.10221696896623202No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12790.10118847005248507No Hit
TCCTGACCCTCCTCACTCACTCTGCAGTGTCAGTGGTCCAGGCAGGGCTG12760.10095112414931272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAA300.002500643767.9640870
ATATATA200.007499318651.503712
TGGTATC3550.043.524262
TCTCGCA805.4662494E-542.47754770
GTATCAA23300.040.8505481
TGTTCTA451.0340043E-438.12674711
GGTATCA9500.037.978671
GCTTGTA853.088644E-936.3829231
GACGGAA750.002036751736.24750570
TATCAAC26450.035.828672
GGCGGTT21900.035.06822270
GTAACCA601.3471279E-534.48322335
TATTTGG800.00279529333.9820470
GGCGTAT6900.033.48954470
AACGCAG28550.033.1722766
ATCAACG28950.032.7191123
TCAACGC29100.032.5453074
CAACGCA29350.032.2680935
TAACCAT652.3199469E-531.8496636
AGAGTAC30600.031.06208811
Produced by FastQC (version 0.11.5)