FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077179
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35750.33188541551589845No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29420.2731208090763002No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26900.24972636859797673No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG26390.24499177945355413No Hit
GTGCAGCCACAGTTCGTTTGATATTCACCTTGGTCCCAGGGCCGAAAGTG25480.23654378705860402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24680.22911698055754892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23560.21871945145607183No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22650.21027145906112168No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22200.2060938804042782No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21810.20247331223501386No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21470.19931691947206545No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCA20590.19114743232090486No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20460.18994057626448344No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGCACCTTTTTAAACTGGTATCAGCAGAAA19880.18455614155121852No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19220.17842902618784806No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACGACATTAGCAC18440.17118788984931937No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18320.17007386887416112No Hit
GTTTGATAGTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTGTC18080.16784582692384462No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18050.16756732168005503No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17810.1653392797297385No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17350.16106886599163184No Hit
CTATCAAACTCTGCCTCTCACTTTCGGCCCTGGGACCAAGGTGAATATCAAACGAACTGTGGCTGCACCAT17350.16106886599163184No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16490.15308504900299766No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCA16460.15280654375920807No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT16030.14881463526489097No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16010.1486289651023646No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15950.14807195461478548No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15880.14742210904594316No Hit
GTTCAGTGGAAGTGGATCTGGGACACATTTTACTTTCACCATCAGCAGCC15850.14714360380215358No Hit
ACCTACTACAATCCGTCCCTCGAGAGTCGGCTAACCATCTCAGTGGACCCGCCCAAGAATCTTTTCTCCCTGAAA15700.14575107758320577No Hit
CACCTGCACTGTCTCTGCTGGCTCCATCAACAGTGCTGATTACTACTGGA15650.14528690217688983No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATTCACCTTGGTCC15620.14500839693310028No Hit
ACATATTACTGTCAACACTATCAAACTCTGCCTCTCACTTTCGGCCCTGGGACCAAGGTGAATATCAAACGAA15500.143894375957942No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15470.14361587071415244No Hit
CCACTGAACCTTGCTGGGACCCCTGTTTCCAAAAAGGATGCATCGCAGAT14730.13674607470067648No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14390.13358968193772808No Hit
ATATTACTGTCAACACTATCAAACTCTGCCTCTCACTTTCGGCCCTGGGA14050.13043328917477967No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13900.12904076295583186No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13720.12736973149309447No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13720.12736973149309447No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCACG13650.12671988592425215No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC13580.12607004035540983No Hit
ATCAAACTCTGCCTCTCACTTTCGGCCCTGGGACCAAGGTGAATATCAAA13450.12486318429898836No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13420.12458467905519881No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13270.12319215283625097No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13190.12244947218614548No Hit
GAGTTTGATAGTGTTGACAGTAATATGTTGCAACATCTTCAGGCTGCAGG13100.1216139564547768No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12980.12049993547961853No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12820.1190145741794075No Hit
GTCTCTGCTGGCTCCATCAACAGTGCTGATTACTACTGGAGTTGGATCCG12480.11585818141645911No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12370.11483699552256403No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12350.11465132536003766No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12300.11418714995372171No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT12250.11372297454740578No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12170.11298029389730027No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11920.11065941686572056No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11830.10982390113435185No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11590.10759585918403533No Hit
ATATTCACCTTGGTCCCAGGGCCGAAAGTGAGAGGCAGAGTTTGATAGTG11440.10620333296508752No Hit
GTAGTAGGTTGTCCCACTGTAATAGATGTAACCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACT11350.10536781723371881No Hit
TCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATGTGTCCCAGATCCACTTCCACTGAACCTTGCTGGGACC11220.10416096117729737No Hit
CTCCTGATCTGCGATGCATCCTTTTTGGAAACAGGGGTCCCAGCAAGGTT11010.10221142447077042No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10900.10119023857687534No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT10860.10081889825182258No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAACG150.002292819669.470946
GTATCAA19050.041.662581
TATCAAC22350.035.511062
TCTTATA501.9069685E-434.3580972
CTTATAC2000.034.3580971
ATCAACG23550.033.849043
TCAACGC24050.033.0024534
GTTATTA800.003253530532.94486670
AACGCAG24350.032.736966
GTGGTAT2750.032.4840241
CAACGCA24550.032.3303075
CTACGCT2100.031.11334428
AGAGTAC26250.030.1000511
CAGAGTA26350.029.85544410
TACGCTG2200.029.70738429
ACTACGC2200.029.69772327
CGCAGAG26750.029.5388018
TATAAGA2100.029.44982
ATAAGAG1900.028.934483
TATACAC2400.028.633083
Produced by FastQC (version 0.11.5)