FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893760
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21960.24570354457572502No Hit
GTAGTACACTGCACAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17750.19859917651271036No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16990.19009577515216614No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11450.12811045470819907No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11230.12564894378804153No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10770.12050214822771213No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10080.11278195488721805No Hit
GTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGA9850.11020855710705335No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9800.10964912280701754No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9780.10942534908700323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9760.1092015753669889No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9420.10539742212674542No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9080.10159326888650197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGC200.00693819952.52540654
TAGACCG200.007531417551.446865
ACGTATA200.007534750351.4411116
GGTATCA8550.044.9282531
GTATCAA20450.041.09041
TATCAAC23800.035.016492
TCGTATG501.8309335E-434.59640
CTCGTAT601.3235334E-534.57063339
TCCTATA807.4707714E-834.295992
TCAACGC25000.033.4728854
ATCAACG25100.033.3395273
AACGCAG25650.032.7620166
CAACGCA25850.032.506725
CGTATGC553.1879247E-431.47755641
CGCAGAG28300.029.6925358
AGAGTAC28750.029.22778111
ACGCAGA28650.029.2100857
ACCGATC1800.028.6200227
ACGAGAC1800.028.5831922
CAGAGTA30350.027.68694110
Produced by FastQC (version 0.11.5)