FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893760
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22350.2500671321160043No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19020.21280880773361976No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17080.19110275689223058No Hit
GTAGTACACTGCACAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16470.18427765843179378No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11430.12788668098818473No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11200.12531328320802004No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10910.12206856426781239No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10790.12072592194772645No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9740.10897780164697457No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9350.10461421410669532No Hit
GTACTACTGTCAACATTATGGTACCTCACCTCGGACGTTCGGCCAAGGGA9300.1040547798066595No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9120.10204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21350.040.787541
GGTATCA8750.038.7899131
ATCAACG24450.035.7143253
TATCAAC24800.035.2221152
TCAACGC24900.034.7918854
CAACGCA25250.034.3096245
AACGCAG25500.033.7046936
CGCAGAG27700.031.2750178
AGAGTAC28000.030.32846611
ACGCAGA28900.029.9764027
CAGAGTA29250.028.91531210
GCAGAGT29850.028.678249
TAATACC500.00802273227.3934564
GTGTAAT907.249011E-626.6653441
TCTAGAC659.040683E-426.3413353
AGTACGG21150.026.06587613
TGTTAGA3407.2759576E-1225.89661670
GAGTACG21650.025.78021412
GTATACA1608.54925E-1125.7130091
TACTAGG951.1100587E-525.2406982
Produced by FastQC (version 0.11.5)