FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192293
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.11754815619992401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92550.042.0747071
GGTATCA35100.041.66371
TATCAAC101250.038.1220172
TCAACGC108750.035.5882034
ATCAACG108950.035.5849343
ACGTAGA5300.034.92700227
CAACGCA110850.034.9147955
GTGGTAT12400.034.2481461
AACGCAG114000.034.040966
AGACGTA5550.033.95509725
CGTAGAG5550.033.36209528
GACGTAG5900.031.36794526
ACGCAGA124000.031.2347767
ACGAGAC6200.030.94157222
CGCAGAG127550.030.338598
CCCACGA6350.029.66442519
AGAGTAC131200.029.59762611
CAGAGTA133650.029.00447510
CGAGACG6500.028.98845923
GCAGAGT135050.028.754519
Produced by FastQC (version 0.11.5)