FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889666
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.041.4643021
GTATCAA23150.041.200391
TATCAAC27450.034.87142
TCAACGC28200.033.8261074
ATCAACG28250.033.7624443
TCGTATG1650.033.55977240
AACGCAG29150.033.078716
CAACGCA29100.033.0176245
CGTATGC1700.032.60229541
TCTCGTA1550.031.1868138
ACGCAGA31900.030.117837
AGAGTAC32400.029.3320411
CGCAGAG33000.029.1139038
CAGAGTA33350.028.60258710
CTCGTAT1750.027.6460639
GCAGAGT35700.027.104249
TACGGGG10650.025.4498415
TATACAC3400.025.2271753
CGAGACG2600.023.75903323
AGTACGG26600.023.7311813
Produced by FastQC (version 0.11.5)