FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446537
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6520.14601253647514092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGG400.0092198448.868870
GTATCAA14750.038.8420261
TATCAAC16700.034.283522
GGTATCA6750.033.5442121
TCAACGC17100.033.4740684
ATCAACG17450.033.002793
CAACGCA17300.032.8889585
AACGCAG18000.031.8003676
ACGCAGA18850.030.548237
CGCAGAG19600.029.3792958
AGAGTAC19450.028.90096711
CAGAGTA19600.028.67978910
GCAGAGT21050.027.192719
GTGGTAT2900.026.025681
AGTACGG16800.025.70400213
GAGTACG16950.025.47938712
TATAAGA1351.00444595E-825.39522
TGGTATC3000.025.1412492
ACGGGTA1251.3429599E-724.67860616
TACGGGC3600.022.85056315
Produced by FastQC (version 0.11.5)