FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102954
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27100.037.2503131
GGTATCA11900.035.778871
GTGGTAT5500.033.711881
TGGTATC5700.033.1313972
ATCAACG32100.031.234153
TCAACGC32300.031.1470574
TATCAAC32650.030.918332
CAACGCA32700.030.8710545
AACGCAG34900.029.1217986
CGAATTA500.00791828127.46769315
ACGCAGA37200.027.2264867
CGCAGAG38000.026.8348
AGAGTAC38250.026.56885311
CAGAGTA38450.026.43065510
GCAGAGT42200.024.2446759
AGTACGG33200.023.68153813
GAGTACG33400.023.64252712
ACGAGAC1653.3141987E-922.89493622
TACACTG2250.022.8907835
TGCGTAC952.742662E-422.15097857
Produced by FastQC (version 0.11.5)