FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1102954
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15510.1406223650306359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6950.035.5537261
GTATCAA27300.035.3249131
TGGTATC7600.030.6927452
ATCAACG32250.029.877193
GGTATCA12300.029.5759141
TCAACGC32800.029.2703324
CAACGCA32700.029.2549865
TATCAAC33100.029.0181982
AACGCAG34050.028.3971986
ATACGAC500.00796906627.4317263
ACGCAGA36800.026.3682987
CGCAGAG37800.025.7614338
AGAGTAC38050.025.05035611
CAGAGTA38550.024.81551610
GCAGAGT40400.023.9337779
GTACGAC1352.8205977E-722.8597723
ACGTGAT1550.003139058121.26712470
GTAGTTT5000.021.09698970
AGTACGG34100.020.9137913
GAGTACG34600.020.7106612
Produced by FastQC (version 0.11.5)