FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49368233
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1359440.2753673602212986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA136300.027.3454511
TCCGATC102950.026.1149276
TCGTATG62800.023.78684844-45
TATGCCG74150.023.44516448-49
ATCGGAA162250.023.0589712
TATCGCG21550.020.49804740-41
CGTATGC87150.020.08395646-47
TTCCGAT134800.020.0503125
TCGGAAG204200.019.144453
CGCGTAT22050.018.63302644-45
CGGAAGA237700.016.6861464
ACGACGC53000.016.48977716-17
CTCGTAT84850.015.92598444-45
GTATGCC124900.015.62998646-47
CCGATCT175650.015.4675197
TATCTCG99800.014.96810740-41
TACACGA100600.014.91976312-13
CTTCCGA189300.014.5788844
ACACGAC107100.013.83687214-15
CTACACG71950.013.83012312-13
Produced by FastQC (version 0.11.5)