FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49368233
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC106700.038.5090566
CCGATCT117150.034.7901427
TTCCGAT156800.026.4774765
CTTCCGA199650.021.341964
CGATCTA34800.021.1557318
TCTTCCG220600.019.1644213
CGATCTG116000.014.4950888
CGACCGT43250.011.3139041
GTCTAAC70650.09.3468221
CGATCTC160150.09.2830988
TCGTAAG43050.09.1583276
CGCGAAT17150.09.1413681
GTCGTAA38700.09.08305555
GACCGTT57100.08.4023612
CGATCTT60000.08.1538288
GCGAACG43150.08.0362473
TACCGTA76900.07.96789079
GCTCTTC603450.07.66007331
ACCGTTG64200.07.54703433
CCGTTGA70450.07.4169084
Produced by FastQC (version 0.11.5)