FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43524680
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2477250.5691598421860885No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTTGTTTATCTCGTAT509980.11717030429632108TruSeq Adapter, Index 8 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG96900.030.21947544-45
TATGCCG129500.026.92228348-49
TCCCTAC145300.026.5439479
CGTATGC136350.025.58693546-47
GATCGGA302450.025.3382951
ATCGGAA317800.024.2032452
ACGACGC79650.023.82352816-17
CTCGTAT113650.023.55060844-45
TATCGCG39900.022.85635240-41
TCGGAAG345950.022.6689433
CTACACG86800.022.18943612-13
TATCTCG134650.022.10005240-41
CGCGTAT37800.021.86427344-45
TACACGA107800.021.72217412-13
GTATGCC179000.021.62649746-47
CGGAAGA365850.021.5916984
ACACGAC114600.020.78554514-15
TTCCCTA206650.018.8704768
TCCGATC101600.017.8126626
GCCGTCT223150.017.75230450-51
Produced by FastQC (version 0.11.5)