FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48091287
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC116600.046.89116
CCGATCT122600.042.7752277
TTCCGAT155150.035.3931925
CGATCTA33700.028.0477228
CTTCCGA210150.026.6500574
TCTTCCG238750.023.4178373
TAGATCG66350.019.32899332-33
CGATCTG111250.019.1272438
GATCGGA208450.015.2014981
ATCGGAA230050.013.752112
AGAGCGT235650.012.8596058
CGATCTC151450.012.0116858
GAGCGTC271950.011.7370319
CGTGTAG141850.011.63632114-15
TCGGAAG298150.010.7702453
GTAGATC130050.010.71993330-31
GCGTCGT158200.010.67373310-11
AAGAGCG292550.010.6191897
GAGTGTA155800.010.19831526-27
GCTCTTC590550.010.0799141
Produced by FastQC (version 0.11.5)