FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44976435
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT775390.17239916858683885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC120050.028.9239436
TTCCGAT143650.024.2382145
TCCCTAC140500.023.5291759
CCGATCT159550.022.0594737
ACGACGC76850.021.7865816-17
TCGTATG58750.021.10123444-45
CTACACG85250.019.4448612-13
GACGTAT81950.019.27167136-37
TACACGA112300.019.11755812-13
ACACGAC115200.018.92491514-15
TATGCCG76950.018.6108148-49
CTTCCGA194300.018.4087544
GATCGGA194700.017.605011
ACGTATC83300.017.39126838-39
ATCGGAA208450.016.4887182
TTCCCTA209300.016.3167428
CGACGCT99650.016.2774816-17
CGTATGC89950.016.2641246-47
TATGACG91100.016.00653634-35
TCGGAAG235300.015.039613
Produced by FastQC (version 0.11.5)