FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005758751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84636390
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG2515600.010.358095140-144
GATCGGA2292450.010.127027140-144
TAGATCG413900.09.299647140-144
ATCGGAA2114700.09.0613785140-144
GAGATCG1477700.08.56088140-144
CAGATCG1026750.08.396677140-144
CCCAAAT883900.08.0778431
CCAAAGT1376700.07.56763122
CCCAAAG1906300.07.38135151
CCCAAAA1060150.07.25819831
CCCTAAA578600.07.22228241
AAGATCG592450.07.165371140-144
CCCAAGT1025500.07.1240541
CCACGAT440550.06.9505411
CCAGAAA1644350.06.92727661
TCGGAAG2152400.06.8996162140-144
CCCTAAT337100.06.77525141
CCCAATT467900.06.74444151
GGCTAAT390550.06.6043681
CCATAAA573050.06.58815341
Produced by FastQC (version 0.11.5)