FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005758792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84576575
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG7577250.09.119219140-144
GATCGGA6968300.09.063671140-144
TAGATCG1277800.08.779288140-144
AGAGCGT4421400.08.734153140-144
ATCGGAA6466550.08.681795140-144
AAGAGCG4794050.08.47957140-144
GAGATCG3578200.08.378626140-144
GAGCGTC4191650.08.366919140-144
CAGATCG2633200.08.239534140-144
AGCGTCG3684150.08.152521140-144
TCGGAAG6230900.07.927164140-144
AAGATCG1473700.07.914123140-144
TAGGGAA2625100.07.4675293140-144
CGGAAGA5966500.07.4505563140-144
GCGTCGT3338050.07.281654140-144
GTAGGGA2746200.07.0879035140-144
TCGTGTA2718350.07.027648135-139
CCAAAGT1265200.06.97246552
CCCAAAT806400.06.96747161
CCCAAAG1851750.06.8695741
Produced by FastQC (version 0.11.5)