FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005758805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80686790
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG6742100.09.852528140-144
GATCGGA6182900.09.773151140-144
TAGATCG1020700.09.40551140-144
ATCGGAA5682700.09.377075140-144
GAGATCG3191400.08.96263140-144
CAGATCG2458600.08.902957140-144
TCGGAAG5451050.08.523969140-144
AAGATCG1365500.08.402483140-144
CGGAAGA5189150.07.904975140-144
AGAGCAC4473900.07.8626585140-144
AAGAGCA5004250.07.4276166140-144
GAGCACA4412000.07.3469872140-144
AGCACAC3855500.07.187654140-144
AATCTCG502250.06.9181085140-144
GAAGAGC5552100.06.890773140-144
GCACACG3266350.06.863096140-144
TCGTATG327600.06.6591616135-139
TCTGAAC2435050.06.562173140-144
TATGCCG341250.06.55313140-144
ACGTCTG2362400.06.4640994135-139
Produced by FastQC (version 0.11.5)