FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005758815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93630941
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGTCATCTCGTAT5770840.6163389941792852TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGTCATCGCGTAT2281850.24370683191147252TruSeq Adapter, Index 14 (97% over 38bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1206790.12888794955077937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)