FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688911
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12670.18391345181017577No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11190.16243027038325705No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8990.1304958115054049No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8980.13035065487414194No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8940.12977002834909007No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8340.12106063047331224No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG8140.11815749784805293No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7630.11075450965364177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7590.11017388312858989No Hit
ATATTACTGTCAGCAGTCTGATAATCTCCCTAGCACTTTTGGCCAGGGGA7510.10901263007848619No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA7170.1040773046155454No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG7020.10189995514660094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18800.039.367131
TCGTATG1300.034.6395840
GTGGTAT3300.033.3802151
TTAACGG1154.5474735E-1133.05143435
TATCAAC23100.032.3370862
CAACGCA23950.031.4791685
AACGCAG24050.031.3482786
TCAACGC24150.031.0759244
ATCAACG24200.031.0117153
TGTTAGA2950.031.0073770
ATTAGAG1251.2732926E-1030.2947483
CAGAGTA25550.029.09942810
AGAGTAC25650.028.9859811
CGCAGAG26450.028.3736698
GCAGAGT26750.028.0534239
TATTAGA1601.8189894E-1227.9689732
CTTATAC1850.027.9108261
ACGCAGA27000.027.7956897
TATAGTG500.00781441727.540685
CGTATGC1653.6379788E-1227.31173741
Produced by FastQC (version 0.11.5)