FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204538
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA92850.42117668191702756No Hit
GTCTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT81920.3715971328232945No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60820.27588546897354455No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT57320.2600091266287993No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45920.20829761156305765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42250.19165013259013908No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36620.16611190190416314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36570.1658850970135239No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36080.16366240908525959No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC35580.1613943601788674No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGACT32810.14882936923745474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32540.14760462282800296No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT32350.14674276424357394No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG32350.14674276424357394No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32330.14665204228731823No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31140.14125408589010488No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA30940.14034686632754798No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG29330.1330437488489652No Hit
CCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTAGTCAGAGCCTCTTG28860.13091178287695654No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA28680.13009528527065536No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26910.12206639214202705No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCC26620.12075092377631957No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCT26620.12075092377631957No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26470.12007050910440191No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26380.11966226030125132No Hit
ATCCATTACTATGCAAGAGGCTCTGACTAGACCTACAGGAGATGTCGGCC26360.11957153834499565No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24710.11208697695390146No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24640.11176945010700655No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT24600.11158800619449516No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24150.10954676217874221No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC22880.10378591795650609No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22750.10319622524084411No Hit
GGTCTAGTCAGAGCCTCTTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC22290.10110962024696331No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22080.1001570397062786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.038.9869581
GTATCAA36650.036.584581
CTTATAC6100.034.2921941
GTGGTAT6550.032.9833341
TATCAAC41350.031.9257492
TGGTATC6650.031.4531462
TCGTATG3600.030.78293640
TATACAC7200.030.0029493
ATCAACG44850.029.96953
CTGTCTT41200.029.96847270
TTATACA7100.029.9425812
CGAGACT6600.029.62203623
TCAACGC45150.029.6184794
CAACGCA46400.028.9683655
GACGTCA6700.028.74887531
AGACTCG6650.028.38063825
AACGCAG47450.028.3273356
ACGAGAC6800.028.24255422
TCGACGT6950.027.19261229
TACACAT7950.027.1724835
Produced by FastQC (version 0.11.5)