FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204538
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA89620.406525085981734No Hit
GTCTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT84370.38271057246461615No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64630.293168001640253No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59170.2684009075824504No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT52810.23955132549314187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39540.17935730751749346No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG38280.17364182427338518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36640.16620262386041884No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36470.1654314872322455No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36460.16538612625411764No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC36210.16425210180092156No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35040.15894486735996385No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG33390.15146030596886967No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA31590.14329532990585783No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT30870.1400293394806531No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGACT30050.13630973927416992No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29830.13531179775535734No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCT28500.12927878766435416No Hit
CCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTAGTCAGAGCCTCTTG27540.1249241337640812No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27020.12256536290143331No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA26340.11948081638873996No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25830.11716740650421992No Hit
ATCCATTACTATGCAAGAGGCTCTGACTAGACCTACAGGAGATGTCGGCC25700.11657771378855797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25460.11548905031348973No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24920.11303955749458618No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG24690.11199625499764576No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23290.10564571805974766No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCC23110.10482922045344648No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC23040.10451169360655158No Hit
GGTCTAGTCAGAGCCTCTTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC23010.10437561067216804No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22870.10374055697837824No Hit
CTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTA22750.10319622524084411No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT22550.10228900567828725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44850.037.7383421
GGTATCA20900.037.0493351
GTGGTAT7450.036.791881
TATCAAC50550.033.203572
CTGTCTT41250.033.13928270
CAACGCA52750.032.3375365
TCAACGC52550.032.197694
AACGCAG52800.032.179366
ATCAACG53150.031.898663
TGGTATC8400.031.3993992
TGTTAGA12650.029.4255770
ACGCAGA58600.028.9963427
AGAGTAC58050.028.97600211
CGCAGAG59800.028.4144768
CAGAGTA59000.028.39330710
AGTACGG52100.026.23520713
GAGTACG52900.025.90380112
CGATTGT1250.001050964125.67127470
TCAAACG32350.023.88746346
ACGAACT33450.023.3985150
Produced by FastQC (version 0.11.5)