FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992461
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17180.17310503888817796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33400.040.5620461
TATCAAC38000.035.646512
GGTATCA13800.035.1325871
GTGGTAT5600.034.99911
TGGTATC5700.033.776732
ATCAACG41700.032.564443
AACGCAG42300.032.102536
CAACGCA42600.031.8764615
TCAACGC42950.031.7767814
AGAGTAC41900.031.4275911
CAGAGTA42100.031.2782910
GCAGAGT43600.030.7542029
GGCTAGT450.00469844730.564521
ACGCAGA44150.030.5268297
CGCAGAG45550.029.6640558
ATAACAC809.793254E-525.7836153
AGTACGG44450.025.67986113
GAGTACG45150.025.12942312
GTGTATA1704.611138E-922.2491721
TACGGGT7300.021.66294715
Produced by FastQC (version 0.11.5)