FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190529
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30860.25921250133344087No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19590.1645487006196405No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19000.1595929204580485No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18810.157996991253468No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC18130.15228524462654836No Hit
GTAATACACGGCCGTGTCCGCAGCAGTCACAGAACTCAGCTTTAGGGAGA18090.1519492595308472No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18060.15169727070907132No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17930.1506053191480426No Hit
GAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT17550.14741346073888162No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16880.1417857103858873No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTT16680.1401057849073815No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16640.13976979981168033No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16290.13682993022429527No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15990.13431004200653657No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15930.13380606436298484No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15520.13036221713204804No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACACCTATTTAAATTGGTATCAGCAGAAA15170.12742234754466292No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15140.12717035872288704No Hit
GTGTATTACTGTGCGAGAGATGTGTGGGCGGTAGGACCAGCTGCGCGAAT14790.12423048913550196No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14490.12171060091774329No Hit
TCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACT14190.11919071269998463No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13360.11221902196418566No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAACAC13280.11154705177278336No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGGTACTTTCTACTGGACCTGGATCCG13230.1111270704031569No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13160.1105390964856799No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC13140.11037110393782933No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12860.10801920826792125No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12640.10617129024156488No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12210.10255945046277748No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12180.1023074616410016No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12100.1016354914495993No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12070.10138350262782345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22750.044.5227851
GTGGTAT3250.041.3425831
TATCAAC26950.037.7072562
ATCAACG28350.035.6006553
CAACGCA28400.035.416695
AACGCAG28650.035.227876
TCAACGC28950.034.862824
GTAATAC4200.034.4521561
CGCTGAT6750.034.00364770
AGAGTAC30700.032.31450711
CGCAGAG31000.032.2240228
AATACAC4600.031.4510293
ACGCAGA32250.031.2954587
GCAGAGT31850.031.2558849
CAGAGTA32100.031.01246310
ATACACG4650.029.6312834
TAATACA4650.028.8917162
GTGTATA2050.026.8894861
GAGTACT11200.025.83477612
AGTACGG28200.025.16294913
Produced by FastQC (version 0.11.5)