FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083764
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1250.055.164521
TAAGACA1400.051.82916331
TATACAC1400.051.7119673
ATAGGTA200.00738034551.711963
TAAGTGG200.00738034551.711965
ATACTTG200.00738034551.711966
TCTTATA200.007383038351.7071882
TTATACA1550.046.703272
ATACACA1700.046.6421624
ACCCTAA1650.045.99590727
CCCTAAG1600.045.28976428
GTGGTAT3450.044.9710771
GTATCAA31800.044.1272771
CCTAAGA1750.041.42309629
GGTATCA11250.041.066921
TGGTATC3850.040.291322
CTAAGAC1800.040.28920730
TATCAAC35200.039.8576282
TACACAT2000.039.645845
GTCATAC350.001352050239.4032331
Produced by FastQC (version 0.11.5)