FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1083764
Sequences flagged as poor quality0
Sequence length20-76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19300.17808305129160962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.055.285441
GTCTAAA200.00739578251.6846420
TGGTATC5400.044.649252
GTATCAA36950.041.493081
GGTATCA14650.039.039171
TATCAAC40650.037.6211662
TTTAGGG400.002526041434.69343642
CTCTTAC501.8762401E-434.4532431
GTGTATA1102.910383E-1134.4532431
TATACCG400.00261832834.440534
ATGGTAC400.002618920334.4389428
CAGAGTA46550.032.2564510
AGAGTAC46800.032.0841411
AACGCAG47450.032.081596
TGTATAA1305.456968E-1231.7941912
CAACGCA48650.031.2902625
GCAGAGT48900.031.1992869
TAAACCC450.004419625330.9493749
TTTTAGG450.00451012430.82142341
TACCATC450.00466175330.6138047
Produced by FastQC (version 0.11.5)