FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences614737
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.041.614411
GTATCAA13000.039.5199741
CTCGTAT459.781706E-538.48391739
TATCAAC15500.033.365522
CAACGCA15850.032.6287425
ATCAACG16150.032.0226363
AACGCAG16450.031.8578196
TCAACGC16650.031.2680684
TATACAC902.0039442E-730.646853
ACGCAGA17600.029.3772377
GTGGTAT2500.028.9636291
GTAAACG605.4450205E-428.72674819
CAGAGTA17400.028.72207310
TGGTATC2550.028.3934042
AGAGTAC17400.028.32590511
CGCAGAG18500.027.7617558
GCAGAGT19050.026.7771159
TTAACGG1057.5650496E-726.3287135
AATCTCG700.001317992224.69122136
GTAGTTT2051.5206188E-624.29597970
Produced by FastQC (version 0.11.5)