FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511701
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31490.20830838902666599No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28610.1892570025421694No Hit
GAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAACTGTGGCTGCACCAT24940.16497971490393934No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24550.16239983965083044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22720.1502942711554732No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTT22440.14844205302503602No Hit
GTAATACACGGCCGTGTCCGCAGCAGTCACAGAACTCAGCTTTAGGGAGA22350.14784669719739552No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21600.14288539863372451No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21560.14262079604366207No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21160.13997477014303755No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19500.12899376265544577No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19440.12859685877035207No Hit
GTGCAGCCACAGTTCGTTCGATTTCCACCTTGGTCCCTTGGCCGAACGTC18280.12092338365854094No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17760.11748354998772906No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16740.11073618394113652No Hit
TCTCTACTGCATCCAATTTGCACTTTGGGGTCCCATCAAGGTTCAGTGGCAGAGGATCTGGGACAGATTTCACT16440.1087516645156681No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAAATCTGTC16320.10795785674548077No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16020.10597333732001235No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15960.10557643343491868No Hit
ACTTACTACTGTCAACAGAGTTACAGTTCCCTCGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCGAACGAA15950.10551028278740307No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15870.10498107760727815No Hit
GTCTCTGGTGGCTCCGTCAGCAGTGGTACTTTCTACTGGACCTGGATCCG15240.10081358681379453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2200.039.3289440
GTATCAA26450.039.1894071
GTGGTAT4650.036.168511
TATCAAC30000.034.6652572
CGCTGAT11800.033.2072170
CGTATGC2750.032.74571241
AACGCAG32200.032.4034166
ATCAACG32300.031.9843143
TCAACGC32250.031.9274794
CAACGCA32850.031.7622535
GCTAATC2550.031.06196633
CTCGTAT2650.030.01237139
AGAGTAC34750.029.71848911
AATCTCG2800.029.57546436
TATACAC3450.028.8504073
ACGCAGA37100.028.1172077
CGCAGAG37400.027.7072548
GCAGAGT37750.027.5385289
CTTATAC3850.026.745331
CAGAGTA39100.026.5877110
Produced by FastQC (version 0.11.5)