FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769412
Sequences flagged as poor quality0
Sequence length20-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21790.28320327730786626No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT10480.13620790941654146No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC10220.13282870555697077No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9210.11970179825633082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4600.038.5922051
TGGTATC4750.038.08482
GTATCAA28650.037.1778341
GGTATCA12200.036.098071
ATAAGGC450.003898670231.7514562
TATCAAC35800.029.9375362
AGAGTAC34850.029.68012211
CAGAGTA35150.029.52392610
AACGCAG36550.029.5138286
CAACGCA37300.028.916635
GCAGAGT36850.028.7177269
ATCAACG37750.028.3006743
GTAGTTT2951.8189894E-1228.26870
CGCAGAG37850.028.1393818
TCAACGC39150.027.55024
TTGTATC500.008142889527.30963117
AGTCATT2155.9280865E-826.85207270
TAGGCAA3850.026.65833570
ACGCAGA40850.026.3235457
TATTAGA2500.025.9407792
Produced by FastQC (version 0.11.5)