FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474376
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30710.2082915077293716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45250.039.300831
GGTATCA19100.036.919031
GTGGTAT8600.035.1976781
TATCAAC53150.033.3208352
TGGTATC9200.033.2670362
AACGCAG57250.030.9956576
CAACGCA59150.029.940875
TCAACGC60250.029.3912434
CAGAGTA57300.029.3481610
ATCAACG60950.029.1110883
AGAGTAC58000.029.05325311
GCAGAGT61650.028.3372239
CGCAGAG61950.028.2555088
ACGCAGA62500.028.171937
TTAGTAC500.00786148927.5088523
GTAGTTT5250.025.62151770
AGTACGG58700.023.72704113
GAGTACG61550.023.01949512
CCGTTAA1954.0017767E-1122.92715316
CCTATAC2155.456968E-1222.3909243
Produced by FastQC (version 0.11.5)