FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415470
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGAGA62110.4387941814379676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43140.30477509237214495No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42930.30329148622012475No Hit
GTGTATTACTGTGCGAAGCCCGGTCCGGATTGTAGTAGTACCAGCTGCTATAATGACTACTGGGGCCAGGGGACC42250.29848742820405944No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA40820.28838477678792207No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT39820.28131998558782595No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39600.2797657315238048No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT39410.27842342119578656No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39320.2777875899877779No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36940.26097338693154926No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG36560.2582887662755127No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33190.23448041993118893No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32580.23017089729913034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30220.2134979900669036No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT29890.21116660897087186No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28680.20261821161875562No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28660.20247691579475371No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27400.19357527888263262No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26810.18940705207457592No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA26020.18382586702650003No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACTAGCAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGGT25590.1807880068104587No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24930.17612524461839532No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24920.17605459670639434No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAACTCAGCTTCAGGGA24910.17598394879439339No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG24830.1754187654983857No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24200.17096794704232515No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24100.17026146792231556No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24060.1699788762743117No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23540.16630518485026174No Hit
CCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTAGTA23400.1653161140822483No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT22960.16220760595420602No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA22820.16121853518619259No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA22530.1591697457381647No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC21930.15493087101810704No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21730.15351791277808782No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21610.15267013783407632No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21570.15238754618607248No Hit
GTCTCCAGCCACCGTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACTAG21400.15118653168205615No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG20970.14814867146601482No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA20730.14645312157799176No Hit
GGATACCAGTGGCCCTGGTGGATGCATGATAGATGAGGACCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGG20710.14631182575398985No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA20670.146029234105986No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20430.14433368421796294No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20270.14320331762594757No Hit
GGCCAAAAGTGATCCAGTTATGATACTGCTGACAGTAATAAACTGCAAAA19950.14094258444191682No Hit
CTCCCAGGGTCCTCATCTATCATGCATCCACCAGGGCCACTGGTATCCCA19770.1396709220258995No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19770.1396709220258995No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19770.1396709220258995No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19630.13868185125788607No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19370.1368450055458611No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19230.13585593477784763No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19220.13578528686584668No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19190.1355733431298438No Hit
GTATCATAACTGGATCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT18980.13408973697782361No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG18890.13345390576981497No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18500.13069863720177752No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT18380.12985086225776596No Hit
ATCATAACTGGATCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA18240.12886179148975252No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT18090.12780207280973813No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC17980.12702494577772755No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17770.12554133962570735No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA17270.12200894402565933No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTACTACTGCTGATGGAG17000.12010145040163336No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC16950.11974821084162858No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16870.11918302754562089No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16730.11819395677760744No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16300.11515609656156613No Hit
GTTATGATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAACT16280.11501480073756419No Hit
GTACTACTACAATCCGGACCGGGCTTCGCACAGTAATACACAGCCGTGTC16270.11494415282556324No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16210.11452026535355747No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15940.11261277172953153No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA15920.1124714759055296No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15630.11042268645750175No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG15620.1103520385455008No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15410.10886843239348062No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAG15330.10830324909747292No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15250.10773806580146522No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC15170.10717288250545755No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT15150.10703158668145564No Hit
ATCATGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCA15110.1067489950334518No Hit
GTATTACTGTGCGAAGCCCGGTCCGGATTGTAGTAGTACCAGCTGCTATAATGACTACTGGGGCCAGGGGACC15070.10646640338544794No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT14980.1058305721774393No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14960.10568927635343738No Hit
CTCCAGCCACCGTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC14730.10406437437741527No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC14340.10130910580937781No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14320.10116780998537588No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC14290.100955866249373No Hit
CCAGTGGCCCTGGTGGATGCATGATAGATGAGGACCCTGGGAGCCTGCCC14270.10081457042537106No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTGGACACGTCG14270.10081457042537106No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAG14250.10067327460136916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAT16650.049.10186470
TTACCAT400.00968013848.28027370
GTATAGG451.9316158E-645.754411
GTATCAA17650.039.6624641
CAAGTAA350.001384824439.21252415
CTTATAC1550.037.6366921
TTATACA1650.037.4341052
CGCTAAT1400.036.8894632
TATACAC1700.036.33313
TATCAAC19500.035.7223742
GGTATCA7450.035.4673421
ATAGTAA400.002474960434.8389748
TCGTATG1750.031.65519140
ATCAACG22150.031.6035123
CTAGACA553.3619677E-431.1950864
ATACTGT553.3619677E-431.1950866
CCACGGA18700.030.97231569
TGTTAGA9150.030.9556670
TCAACGC22650.030.9058634
AACGCAG22700.030.8377866
Produced by FastQC (version 0.11.5)