FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141269
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGCCTCCATATGCGCAGCAATAATAAT68730.3209778874116237No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGG68310.3190164337129057No Hit
GTGTGGAGGTCTCCACTCCCGCCTGGACGGGGCTGCTATCTGCCTTCCAG68060.3178489017493832No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGCCTCCATATGCGCAG64450.30098974019611735No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC64320.3003826235750856No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51900.24237963562728457No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC48860.22818244695085016No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG44540.20800749462118023No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG43710.20413128850228532No Hit
CCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC43250.20198302968940382No Hit
TCTCTGGGCTCCAGGGTGAAGATGAGGCTGATTATTATTGCTGCGCATAT43190.2017028220181584No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGT42340.19773321334218166No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG41380.193249890602255No Hit
CCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39890.18629140009966053No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39690.1853573745288425No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC39480.1843766476794835No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCACCATTTCCTG37690.17601711882066196No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36360.1698058487747219No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC35200.16438850046397718No Hit
TAGCAGCCCCGTCCAGGCGGGAGTGGAGACCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG34060.1590645547103143No Hit
GACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCACCATTT33910.15836403553220077No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCAC33090.15453453069184675No Hit
GTTTGGAGGGTGTGGAGGTCTCCACTCCCGCCTGGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32940.15383401151373322No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32590.15219946676480162No Hit
GCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCAC31740.14822985808882488No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31620.14766944274633406No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGAGGTCTCCA31070.14510087242658443No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30760.14365313279181643No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC30370.14183178292872123No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT29230.13650783717505835No Hit
CACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28730.13417277324801322No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGC28620.1336590591840633No Hit
CGCCTGGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC28400.13263163105616343No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27830.12996965817933198No Hit
CTCTTGGACAATCAGTCACCATTTCCTGCACTGGAACCAGCAGTGATGTT27520.128521918544564No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCC27320.12758789297374593No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG27310.12754119169520506No Hit
ACAATACCCAGGCAAAGCCCCCAAACTGCTGATTTTTGATGTCAGTAGGCGGCCCTCAGGGGTCCCTGATCGCTT27230.12716758146687782No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT27160.1268406725170915No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26420.1233847779050647No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC25880.12086290886385598No Hit
GGTATCAACAATACCCAGGCAAAGCCCCCAAACTGCTGATTTTTGATGTC25830.12062940247115146No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGA25460.11890145516513806No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGC25200.11768722192307458No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24760.11563236566727486No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC24250.11325060046168883No Hit
GTCCAGGCGGGAGTGGAGACCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGC24220.11311049662606613No Hit
CAACAATACCCAGGCAAAGCCCCCAAACTGCTGATTTTTGATGTCAGTAGGCGGCCCTCAGGGGTCCCTGATCG24200.11301709406898433No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23510.10979470584966204No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23410.10932769306425302No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23200.10834696621489406No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATC23160.10816016110073046No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22660.10582509717368532No Hit
CCCCTGAGGGCCGCCTACTGACATCAAAAATCAGCAGTTTGGGGGCTTTGCCTGGGTATTGTTGATACCAGGAGA22580.10545148694535811No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGT22450.10484437032432638No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22300.10414385114621283No Hit
GTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC21910.10232250128311762No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGAGGTCTCCACTCCCGCCTGGA21590.10082806036980875No Hit
ATGTATTACTGTGCGAGAGTGAAGAATATCTATACCGACAGTTGGTTCTG21570.10073465781272695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3650.043.6551540
CGTATGC4050.040.22908841
TATCGTA557.2619005E-637.73788538
CTCGTAT3250.037.27944639
ATCAACG61300.033.4601063
TCAACGC61600.033.408894
CAACGCA62700.032.822775
CGAGACA5700.032.60778823
AACGCAG63350.032.5403186
TATGCCG4750.032.14370743
CTTATAC5300.031.1693121
AATCTCG3900.031.01738436
TATACAC6050.030.7170833
CGTCTAG450.004678958630.5921041
ACGAGAC5450.030.31144322
TCTCGTA3900.030.15805238
AGAGTAC67050.030.07178911
CGCAGAG68150.029.9419258
TTATACA5650.029.8462122
CAGAGTA67900.029.69533710
Produced by FastQC (version 0.11.5)