FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005758884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141269
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGCCTCCATATGCGCAGCAATAATAAT72120.3368096208369897No Hit
GTGTGGAGGTCTCCACTCCCGCCTGGACGGGGCTGCTATCTGCCTTCCAG68550.3201372643978874No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGCCTCCATATGCGCAG67990.31752199279959686No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC65000.303558310515867No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGG60300.28160870960164275No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC52140.24350046631226624No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC45830.2140319595529567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45680.21333144037484314No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC44450.20758718311431212No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG43170.20160941946107658No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG42010.19619207115033188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG40780.19044781388980087No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT40690.19002750238293273No Hit
TCTCTGGGCTCCAGGGTGAAGATGAGGCTGATTATTATTGCTGCGCATAT39730.18554417964300607No Hit
CCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC39290.18348932338720636No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGT39000.18213498630952019No Hit
CCTCTGAGGAGCTTCAGGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37340.17438257407173036No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCACCATTTCCTG36720.1714870948021944No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCAC36330.1696657449390992No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC34910.16303416338629104No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34660.16186663142276847No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGG32940.15383401151373322No Hit
GCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCAC32690.15266647955021065No Hit
GTTTGGAGGGTGTGGAGGTCTCCACTCCCGCCTGGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32370.15117203863690176No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG32310.15089183096565636No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31590.14752933891071135No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC30970.1446338596411754No Hit
TAGCAGCCCCGTCCAGGCGGGAGTGGAGACCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG30860.14412014557722547No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGAGGTCTCCA30540.14262570466391658No Hit
GACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATCAGTCACCATTT29860.1394500177231352No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29270.13669464228922196No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGC27790.12978285306516837No Hit
CACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27760.12964274922954566No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27420.12805490575915499No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT26940.12581324438919164No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26000.12142332420634679No Hit
CGCCTGGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC25290.11810753342994271No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24350.11371761324709785No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC24300.11348410685439335No Hit
GGTATCAACAATACCCAGGCAAAGCCCCCAAACTGCTGATTTTTGATGTC24250.11325060046168883No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24090.1125033800050344No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCACGCGACCCCGAGAGCCT23890.11156935443421634No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23830.11128914676297093No Hit
GTCCAGGCGGGAGTGGAGACCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGC23660.11049522502777559No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGA23550.10998151096382566No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGAGGTCTCCACTCCCGCCTGGA23530.10988810840674385No Hit
ACAATACCCAGGCAAAGCCCCCAAACTGCTGATTTTTGATGTCAGTAGGCGGCCCTCAGGGGTCCCTGATCGCTT23210.10839366749343497No Hit
GTGTGGCCTTGTTGGCCTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC22810.10652561635179886No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCC22660.10582509717368532No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGAGGGCCGC22600.10554488950243991No Hit
CTCTTGGACAATCAGTCACCATTTCCTGCACTGGAACCAGCAGTGATGTT22470.10493777288140818No Hit
CCCCTGAGGGCCGCCTACTGACATCAAAAATCAGCAGTTTGGGGGCTTTGCCTGGGTATTGTTGATACCAGGAGA22440.10479766904578547No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT22230.10381694219642652No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC22170.1035367345251811No Hit
CCTTGGTCCCTCCGCCGAACACGAGAGACCTGCTGCCTCCATATGCGCAGCAATAATAATCAGCCTCATCTTCAC21890.10222909872603582No Hit
CAGTCTGCCCTGACTCAGCCTCGCTCAATGTCCGGGTCTCTTGGACAATC21750.10157528082646318No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21680.10124837187667687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)