FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084161
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81020.38874156075274413No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52350.251180211125724No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC50460.24211181381860614No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50190.24081632848901788No Hit
GTCTCCAAACCGTGTTCACTTTTGGCCAGGGGACCAAGCTGGTGATCAAA50000.23990469066449283No Hit
GTTTGGAGACCTTGCATGCAGTAATAAACCCCAGCATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT49270.23640208218079123No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48790.2340989971504121No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC48380.23213177868696327No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48070.23064436960484339No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47790.22930090333712225No Hit
GTGCAGCCACAGTTCGTTTGATCACCAGCTTGGTCCCCTGGCCAAAAGTG47670.22872513207952747No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT46930.22517454265769293No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45970.2205683725969347No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC45710.2193208682054793No Hit
GTAATAGACGGCCGTGTCGTCGGCTCTCAGGCTGTTCATGTGAAGATACA45050.216154126288708No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT42850.20559831989947036No Hit
GGTCTAGTCAGAGCCTCCTGCATAGGAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC42120.20209571141576876No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA40890.1961940560254222No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39850.19120403845960077No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCT38970.1869817159039057No Hit
GTAATAAACCCCAGCATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA38440.18443872618286206No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37290.17892091829757875No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35870.17210762508270713No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34700.166493855321158No Hit
GTCTATTACTGCGCGAGAGGATCAACAGTGCCTGGTAGTGGGTATTACAC34610.16606202687796193No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34260.1643826940433105No Hit
CTCCCTGCCCGTCACCCCTGGCGAGCCGGCCTCCATCTCCTGCAGGTCTA32870.15771334364283757No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA32420.15555420142685714No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32230.15464256360233208No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31640.15181168825249106No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC31610.15166774543809236No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31360.1504682219847699No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA30640.1470135944392012No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG30560.146629746934138No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC30520.14643782318160642No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA30400.14586205192401164No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC30040.1441347381512273No Hit
CTCCTGTGCAGCCTCTGAATCCACCGGCAGTAGCACCTACATGACCTGGGTCCGGCAGGCTCCAGGGAAGGGT29400.14106395811072175No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28970.13900077777100714No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG28860.13847298745154527No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAATCGGGCC28670.13756134962702019No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA28510.1367936546168938No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28490.136697692740628No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT28440.1364577880499635No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT28130.13497037896784367No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28040.13453855052464755No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC27680.13281123675186324No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC27610.1324753701849329No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27410.13151575142227495No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT27350.13122786579347756No Hit
GGTCTGGAGTGGGTCTCAGTTATTTATAGTGCTGGTAGCACATACTACGC27130.1301722851545538No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26500.1271494860521812No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC26000.12475043914553627No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCACCAGCTTGGTCC25650.1230711063108848No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC25630.12297514443461902No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25590.12278322068208741No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25550.12259129692955584No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT25340.12158369722876496No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25300.12139177347623335No Hit
GTCTCCACAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCC24840.11918465032212003No Hit
GTGTAATACCCACTACCAGGCACTGTTGATCCTCTCGCGCAGTAATAGACGGCCGTGTCGTCGGCTCTCAGG24480.11745733654933568No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCTGA24440.11726541279680408No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24420.11716945092053829No Hit
CTCCAGGGAAGGGTCTGGAGTGGGTCTCAGTTATTTATAGTGCTGGTAGC24310.11664166060107642No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA24160.11592194652908293No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT24150.11587396559095003No Hit
CCCCTGGCGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG23750.11395472806563409No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23610.1132829949317735No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23410.11232337616911554No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23290.11174760491152073No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA23270.11165164303525495No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT23100.11083596708699568No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22860.10968442457180612No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22830.10954048175740741No Hit
CAGTAATAAACCCCAGCATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA22720.10901269143794552No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGG22420.10757326329395858No Hit
CTCCACAGCTCCTGATCTATTTGGCTTCTAATCGGGCCTCCGGGGTCCCT22320.10709345391262959No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA22290.1069495110982309No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22280.10690153016009801No Hit
GTGGAGGCTGAGGATGCTGGGGTTTATTACTGCATGCAAGGTCTCCAAAC22210.1065656635931677No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA22090.10598989233557292No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA22080.10594191139744003No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21990.10551008295424393No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21830.10474238794411757No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21410.10272718854253582No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG21220.10181555071801075No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGAGGAGGCTTGATCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC21200.10171958884174495No Hit
TTATTACTGCATGCAAGGTCTCCAAACCGTGTTCACTTTTGGCCAGGGGA21190.10167160790361204No Hit
GCATAGGAATGGAAACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGC21100.10123977946041597No Hit
CTTTTGGCCAGGGGACCAAGCTGGTGATCAAACGAACTGTGGCTGCACCA21070.10109583664601728No Hit
GGCCAAAAGTGAACACGGTTTGGAGACCTTGCATGCAGTAATAAACCCCA20960.10056804632655539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5450.041.03421
TGGTATC5600.040.5387542
GGTATCA13450.037.6031461
GTATCAA30250.037.5333821
TATCAAC34300.032.792022
TGTTAGA12450.031.15740470
ATCAACG36350.031.1296833
AACGCAG36900.030.855816
TCAACGC36950.030.6241954
TATAAGA2250.030.5746842
CAACGCA37250.030.4720845
AGAGTAC39050.028.71790311
ATAAGAG2300.028.4124723
CGCAGAG39900.028.3654448
CAGAGTA39950.028.1570510
GTATAAG2850.027.765921
TATATAC756.293316E-527.5172162
ACGCAGA44300.025.7034197
GTATATA951.0738822E-525.3514961
TTCGGGG21350.024.42399870
Produced by FastQC (version 0.11.5)