FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842461
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9090.10789816976690908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG605.4496923E-946.14999440
GTATCAA15000.038.5289731
GGTATCA6950.038.113191
ACTAGAT807.1106115E-834.48890332
ACCGTAC807.230483E-834.42334727
TATCAAC17550.032.5348472
GTGGTAT2600.031.7546441
AACGCAG18600.031.2529936
CAACGCA18700.030.9019225
CGTACTA902.0186053E-730.62399529
ATCAACG18900.030.5749193
TCAACGC18900.030.5749194
AGAGTAC20550.028.62044111
ACGCAGA20750.028.1804987
CAGAGTA21200.027.90516710
GTACTAG1005.068505E-727.56651330
GTATATA756.283526E-527.5206931
TGGTATC2900.027.2802072
CGCAGAG21750.027.0413828
TGTTAGA2451.0524673E-825.28461570
Produced by FastQC (version 0.11.5)