FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842461
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14200.16855379655556754No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9070.10766077005345054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17150.036.492281
GGTATCA8350.034.592871
CGTAACG400.002605954234.4729232
TATCAAC20050.030.6886442
AACGCAG20400.030.6676336
CAACGCA20550.030.1092385
TCAACGC20450.030.0883774
ATCAACG20700.029.5589313
GTGGTAT3300.029.1768111
AGAGTAC22250.028.42841311
CAGAGTA23150.027.47007210
ACGCAGA22900.027.169537
TGGTATC3550.026.1441462
CGCAGAG23700.026.1073768
AGTACGG23600.024.47158613
GAGTACG24100.024.2491612
GCAGAGT28550.022.7559389
GTATGTA3550.022.2788731
TACGGGG12000.021.77581815
GTACGGG26950.020.53675814
Produced by FastQC (version 0.11.5)