FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1305571
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33050.25314594150758557No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA32280.24724813893690958No Hit
GTGTATTACTGTGCGAGACCAATACCAGCTGCTATTGGTCCTTATCAACGCGATGATGCTTTTGATATCTGGGGC26950.20642308997365902No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAGCA26290.20136783062736535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25870.19815084740699662No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24810.19003179451749463No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23660.1812233880807708No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT23620.18091700872644995No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23370.1790021377619448No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT23310.17854256873046354No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22390.17149584358108444No Hit
GTCTATTATAGCGGAAGCACCTACTACAACCCGTCCCTCAAAAGTCGGAT22320.170959679711023No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21330.16337679069158245No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21040.16115554037275645No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTCACTACTGGGGCTGGCTCCG20990.16077256617985541No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20200.15472157393201902No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20190.15464497909343883No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC18950.14514721910949308No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTTTGATAACTGG18830.1442280810465306No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18700.1432323481449879No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18540.1420068307277046No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17550.13442394170826405No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17480.1338877778382026No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT17160.13143674300363595No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16300.1248495868857381No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA16110.12339428495271418No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16060.12301131075981314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15800.12101984495672775No Hit
ATAATAGACAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCCGGCGGAGCCAGCCCCAGTAGTGACTACTA15660.11994751721660483No Hit
CCATAGATGAGGAGCCTGGGAGCCTGCCCAGGTTTCTGCTGGTACCAGGC15130.11588799077185384No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14820.1135135507758674No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14720.11274760239006534No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14630.11205824884284347No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14530.1112923004570414No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14410.11037316239407892No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14260.1092242398153758No Hit
CTACTGGGGCTGGCTCCGCCGGCCCCCAGGGAAGGGGCTGGAGTGGATTGGGA14020.10738596368945082No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGTAACTTAGCCTGGTACCAGCAGAAACCTGGGCAGGCTCCCAGGCT13680.10478173917772378No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA13550.10378600627618108No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13440.1029434630517988No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13370.10240729918173734No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAGCACTGGAGGAATACTG13330.10210091982741651No Hit
CTCCAGGCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC13310.1019477301502561No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13190.10102859208729362No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13130.10056902305581236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATT3750.048.406221
TCTATTA3750.048.39882
TATAGCG3900.048.2915887
TGTCTAT1700.045.61770270
TATTATA4100.045.102434
CTATTAT4300.043.0046423
ATTATAG4600.040.199995
GGTATCA9550.039.4498981
TAGCGGA4700.039.341669
TTATAGC4750.038.930526
GTATCAA21600.038.3723341
TACAGAT12400.037.52424270
AGTATCG905.2805262E-934.4396634
ATAGCGG5500.033.6205258
AGCGGAA5500.033.61923610
TTATACA1950.033.3663752
TATACAC2200.032.687873
ATAGTAC553.4024083E-431.1313063
ATCAGTA2450.029.46409631
AACGCAG28300.029.2832286
Produced by FastQC (version 0.11.5)