FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678146
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT22540.33237680381510765No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17720.2613006638688424No Hit
GTATCATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16400.24183582886281124No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15670.23107118526099096No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14590.21514541116514735No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13770.20305361972200678No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13100.19317374134773338No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12710.18742276736867872No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12330.18181925426088186No Hit
GCATAGATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC12260.1807870281620772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11740.17311906285667097No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11530.17002238456025692No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10750.15852043660214762No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10730.158225514859632No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTC10320.15217961913806172No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCA9890.1458388016739758No Hit
ATACTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA9340.13772845375479614No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9300.13713861026976493No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9120.13448431458712432No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8900.13124017541945246No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8860.1306503319344212No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA8800.12976556670687434No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8670.1278485753805228No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8620.12711127102423372No Hit
GGTTTATACTACTGTGCGAGAATGCAGGCCTGGGGGGGGGTGGGTTACCC8410.12401459272781967No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8310.12253998401524156No Hit
GCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT8190.1207704535601478No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGGTCCCTGATCTATGCTGCATC8080.11914838397631188No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7920.1167890100361869No Hit
GATCAGGGACCTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC7870.11605170567989782No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7850.11575678393738222No Hit
CAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT7840.1156093230661244No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7730.11398725348228847No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7700.11354487086851503No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7640.11266010564096816No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7590.1119228012846791No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7560.11148041867090566No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7540.11118549692839005No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7420.10941596647329631No Hit
GGTAAGTATCATACTGTTGGCAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT7390.10897358385952287No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7350.10838374037449162No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7320.10794135776071818No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7280.10735151427568694No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7230.10661420991939789No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG7230.10661420991939789No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6920.10204292291040573No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAG6890.10160054029663228No Hit
GGACACAGCATGGACATGAGGGTCCTCGCTCAGCTCCTGGGGCTCCTGCT6790.10012593158405417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC1050.052.342183
TTATACA1000.051.5243382
CTCGGTT305.938821E-446.56868753
TATACAG306.446962E-445.7994085
CTTATAC1150.044.8070761
TCGTATG950.043.7470240
CGTATGC856.366463E-1140.76902841
GTGGTAT2000.039.5049061
GTCGTAT459.804005E-538.46973439
GTATCAA9700.036.4769631
GAGCGAT1450.035.66289532
CTCGTAT709.461746E-734.6227639
AATGGTA901.8423672E-434.552970
ATACACA1550.033.241514
GTCCTAA553.3386878E-431.2291741
TCAACGC11300.031.0057964
CGTATGG450.0045219530.80326341
CGAGACA1800.030.55321723
GTATGCC1251.1823431E-1030.51339142
CAACGCA11600.030.203925
Produced by FastQC (version 0.11.5)