FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653595
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATAA100.009844888129.9121970
CTAGGTC150.00225783169.7376751
ATTAGGG150.002360734268.958363
GCTAGGT200.007071922552.27088550
ACATTTA200.007220179351.99736439
TTACTCT200.007375200351.7187735
CTACCCA200.007375200351.7187734
CTAGGAG400.00933754148.71706870
CTTATAC451.8688934E-645.9687231
GTGGTAT2250.044.436431
GTATCAA18100.043.2385371
GGTATCA6800.042.5886731
TGGTATC2350.042.548782
TTATACA503.856212E-641.3750152
GAGCCTA702.1704182E-839.46216631
CTCTGTA350.001353430639.392727
TATCAAC19800.039.3550262
TTAGGGC400.002603212634.479184
TATACAC709.773721E-734.479183
AACGCAG24150.032.6945466
Produced by FastQC (version 0.11.5)