FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653595
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13560.20746792738622544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTAT208.539172E-568.9546720
GTTAGTA208.561858E-568.9177515
TTAGTAA208.561858E-568.9177516
CGTTAGT208.565102E-568.91247614
AACTATT252.5747047E-455.16374221
TAGTAAC252.5805525E-455.13841617
TATAGTT200.00715378352.1189845
GTGGTAT2450.052.067821
TAGGTAT200.00731443151.827131
TAATTGC200.007374523751.7199722
TGGTATC2450.050.6450732
GGTATCA7950.045.1024321
GTATCAA20150.041.5781361
ATAGTGG350.001289332439.78336349
AAAATAG350.001298176739.72809246
TATCAAC22200.038.1929242
TGGCTAA400.002402157535.04883261
GTGTATA400.002603893634.4773371
GTAACTA400.002606827834.4694219
TGTATAA400.002607806434.4667852
Produced by FastQC (version 0.11.5)