FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences858904
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGT200.0072893251.87359236
CTTATAC408.350398E-751.6711461
TTATACA408.3638406E-751.659112
GTATCAA26800.043.059291
GGTATCA10700.041.530081
AAAGTTT350.001232741440.14959361
TATCAAC30200.038.2026522
CTAATCT557.3102E-637.69759434
TAAGATG557.4580166E-637.58777627
TATACAC557.481902E-637.5702633
TAGTGAC400.002545237534.63910739
TAATCTC601.3225164E-534.57430635
CAGCTAA601.33955655E-534.50974731
GTGGTAT3200.034.4474331
TGGTATC3300.033.395792
AACGCAG36100.032.1498036
AGAGTAC35550.031.57053611
CAGAGTA35600.031.52619410
ATCAACG37150.031.0557233
AGGAGTA1001.474109E-830.9954662
Produced by FastQC (version 0.11.5)