FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672679
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT150.002378484568.8284411
TTATACA501.6370905E-1161.9548032
CTTATAC501.6370905E-1161.9548031
TATACAC501.6370905E-1161.9548033
TCTATTA200.00423604659.49809369
AAGCGTA350.00522100956.43277470
GATAAAC200.007139983652.14459645
ATACACA601.0004442E-1051.6294
AGACTAG605.7079887E-945.90609725
TGTATAA605.7261786E-945.88903414
GTGTATA605.7261786E-945.88903413
TGTGTAT651.1616066E-842.35910812
GTATCAA20000.041.9915921
GTGGTAT2450.040.7412571
GAGACTA702.2309905E-839.3393124
ATGTGTA702.235356E-839.33053611
TATCAAC22000.038.174172
GGTATCA8100.037.818781
ACTAGCG807.177732E-834.4500727
GACTAGC807.2059265E-834.43469626
Produced by FastQC (version 0.11.5)