FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672679
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10260.15252445817395816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGTAA200.0074350151.61338423
TATCTAA400.00867635949.63109670
GTATCAA19400.043.6124731
GTGGTAT2150.041.5922281
TATCAAC21200.040.2310032
GGTATCA7150.039.925411
CAGAGTA24550.033.33790210
TGGTATC2800.033.162772
AACGCAG25800.033.055586
AGAGTAC25000.032.73781611
CAACGCA26600.032.319985
GCAGAGT26250.032.2269029
GTATAAG553.3151172E-431.2668881
TATAAGA553.3165628E-431.2645632
ATAAGAG553.318009E-431.2622383
ATACCAC553.318009E-431.2622387
ATCAACG27700.031.1606663
TTCCATA450.00469341830.5697962
ACGCAGA27950.030.512847
TCAACGC28250.030.1888124
Produced by FastQC (version 0.11.5)