FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561036
Sequences flagged as poor quality0
Sequence length20-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGA300.00311035364.31560570
TAAGTAG200.0074168151.6442433
TATACAC1350.048.2446673
CTTATAC1250.046.6237371
TTATACA1350.043.166282
AGGGGGG1408.785719E-1041.34574570
GTATCAA16700.039.414221
ATACACA1500.038.8496554
GGTATCA7250.037.3557321
TCGTATG1151.8189894E-1236.13022240
TACACAT1900.036.0832675
GGATAAA400.00267707534.282161
TATCAAC19500.033.7517362
CGTATGC1450.033.46096441
ACACATC1950.033.4001546
GTGGTAT2700.031.7427431
AACGCAG20900.031.6548676
CAACGCA21600.030.6290155
GATAAAG450.004768176530.4703142
TCTTATA450.004768176530.4703142
Produced by FastQC (version 0.11.5)