FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005758902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005758902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561036
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15730.2803741649377224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG350.001327098939.54943540
TACTATG350.001394819839.1510855
GTGGTAT3050.038.205381
GGTATCA9300.037.5891651
GTATCAA20400.037.464521
TGGTATC3350.034.76542
GTCTAGG501.9339536E-434.2724721
ACTATCC400.0026854834.2602548
TCCTAGG400.002687828834.2541472
TATCAAC22700.033.6505472
TTAATAG450.00409409431.43430160
ATTATCC450.00478519430.448133
AACGCAG25550.030.0363856
CAGAGTA25500.029.28915410
AGAGTAC25650.028.98430411
CAACGCA26900.028.653795
GCAGAGT26850.028.3269149
ATCAACG27600.027.9245763
ACGCAGA27650.027.7551387
CGCAGAG27800.027.4821438
Produced by FastQC (version 0.11.5)